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murine lymphatic endothelial cell line  (ATCC)


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    ATCC murine lymphatic endothelial cell line
    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Murine Lymphatic Endothelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+lymphatic+endothelial+cell+line/SVEC4-10/pm34799115-41-0-7
    Average 96 stars, based on 383 article reviews
    murine lymphatic endothelial cell line - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Chemical interactions among caseins during rennet coagulation of milk."

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.

    Journal: Journal of dairy science

    doi: 10.3168/jds.2021-21071

    Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Functional Assay, Immunofluorescence, Staining, Labeling, Imaging, Tube Formation Assay, Wound Healing Assay, Migration, Positive Control

    Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Staining, Fluorescence, Injection

    Related Articles

    Modification:

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.
    Article Snippet: Human umbilical vein endothelial (HUVEC) cells (Lonza, C2517A) were grown and maintained in Endothelial Cell Growth Medium-2 (EGM-2, Lonza, CC3162). .. Murine lymphatic endothelial cell line (SVEC4-10, CRL-2181 ATCC) were grown and maintained in high (4.5 g/L) glucose containing Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher) supplemented with 10% heat-inactivated exosome-depleted fetal bovine serum (FBS; Thermo Fisher). ..



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    murine lymphatic endothelial cell line  (ATCC)
    96
    ATCC murine lymphatic endothelial cell line
    Fig. 2. Functional assessment of Az-EVs in 2D <t>endothelial</t> culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
    Murine Lymphatic Endothelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+lymphatic+endothelial+cell+line/SVEC4-10/pm34799115-41-0-7
    Average 96 stars, based on 1 article reviews
    murine lymphatic endothelial cell line - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

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    Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Journal of dairy science

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.

    doi: 10.3168/jds.2021-21071

    Figure Lengend Snippet: Fig. 2. Functional assessment of Az-EVs in 2D endothelial culture. (A,B) Immunofluorescence staining (A) and quantification (B) of EV cellular uptake by HUVECs. MSC-EVs with Ac4ManNAz labeling with and without neuraminidase post-labeling treatment and MSC-EVs without Ac4ManNAz labeling (DMSO) were stained with lipophilic dye (PKH26) and DBCO-AF488. Cells were co-stained with Phalloidin and DAPI (n = 9). (C–E) Brightfield imaging (C) and quantification of tube length (D) and number of branch points (E) of HUVECs tube formation assay over 6 and 12 h culture periods. Cell were exposed to 0, 5 or 10 μg/mL Az-EVs (AE) or non-Az-EVs (NE) (n = 3). (F,G) Brightfield imaging (F) and quantification (G) of endothelial scratch wound healing assay using HUVECs and SVECs over 6 and 12 h culture periods. Cell were exposed to 0 or 20 μg/mL Az-EVs (n = 3). (H,I) Imaging (H) and quantification (I) of endothelial transwell migration using HUVECs and SVECs when exposed to varying concentrations of Az-EVs (5, 10, and 20 μg/mL). As a positive control, cells were treated with medium supplemented with FBS and 10 ng/mL epidermal growth factor (EGF) (n = 3). One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Murine lymphatic endothelial cell line (SVEC4-10, CRL-2181 ATCC) were grown and maintained in high (4.5 g/L) glucose containing Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher) supplemented with 10% heat-inactivated exosome-depleted fetal bovine serum (FBS; Thermo Fisher).

    Techniques: Functional Assay, Immunofluorescence, Staining, Labeling, Imaging, Tube Formation Assay, Wound Healing Assay, Migration, Positive Control

    Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Journal of dairy science

    Article Title: Chemical interactions among caseins during rennet coagulation of milk.

    doi: 10.3168/jds.2021-21071

    Figure Lengend Snippet: Fig. 5. Assessing host vascular ingrowth and macrophage recruitment in EV-functionalized collagen implants. (A,B) Histological analysis (A) and quanti fication (B) of host vascular ingrowth via endothelial CD31 staining (n = 7). (C,D) Histological analysis (C) and quantification (D) of M1 macrophage staining (CCR7) (n = 7). (E,F) Histological analysis (E) and quantification (F) of M2 macrophage staining (MMR) (n = 7). (G,H) IVIS images (G) and the quantification of fluorescence intensities (H) of the lectin accumulation in the implants 20 min after i.v. injection (n = 4). (I) Representative images for lectin perfused vasculature in the implants. One-way ANOVA was used for statistical analysis and quantitative data were displayed as with mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Murine lymphatic endothelial cell line (SVEC4-10, CRL-2181 ATCC) were grown and maintained in high (4.5 g/L) glucose containing Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher) supplemented with 10% heat-inactivated exosome-depleted fetal bovine serum (FBS; Thermo Fisher).

    Techniques: Staining, Fluorescence, Injection